Anti reflux formula

Could anyone help me with preparing anti reflux formula I use a flask for nighttime and I am wondering should I leave the kettle to cool for 45 mins before putting it in the flask or put it straight in from boiled. Any advice from mummies who have used this formula would be appreciated I’m tearing my hair out
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I do hot shot method so babies on 4oz so i pour in 2oz boiled from the flask then the 4 scoops of powder give it a shake to sterilise the powder in the boiled water then add the remaining 2oz of cool boiled water and i use the reflux milk too x

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